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Automated bioreactor for the cultivation of living cells
Ukropcová, Iveta ; Tolde, Ondřej (referee) ; Dostál, Zbyněk (advisor)
Control of cultivation conditions in the~live cell imaging extends the possibilities of biological experiments and makes the experimental results more reliable. In order to change the~cultivation conditions in a controlled manner and increase the reproducibility of the experiments, it is necessary to reduce the amount of manual operations and replace them with automated procedures. Therefore, the concept of a new automated culture device (bioreactor) was created. This device controls the exchange of medium in the observation chamber, ensures the circulation and exchange of the atmosphere and controls its composition. The bioreactor is intended for use in the Laboratory of Experimental Biophotonics. This laboratory is equipped with coherence-controlled holographic microscope (CCHM), which uses quantitative phase imaging (QPI) method. Thus, the bioreactor is adapted to the current requirements of this laboratory and optical elements of the bioreactor meet the requirements of the QPI method. This text specifies the cultivation conditions of the living cells and summarizes, how the conditions could be controlled in the live cell microscopy. Next some commercially available culture devices are described and assessed, whether they are convenient for the~use in Laboratory of Experimental Biophotonics. The crucial part of the thesis is the~design, construction and testing of the new bioreactor.
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Automated bioreactor for the cultivation of living cells
Ukropcová, Iveta ; Tolde, Ondřej (referee) ; Dostál, Zbyněk (advisor)
Control of cultivation conditions in the~live cell imaging extends the possibilities of biological experiments and makes the experimental results more reliable. In order to change the~cultivation conditions in a controlled manner and increase the reproducibility of the experiments, it is necessary to reduce the amount of manual operations and replace them with automated procedures. Therefore, the concept of a new automated culture device (bioreactor) was created. This device controls the exchange of medium in the observation chamber, ensures the circulation and exchange of the atmosphere and controls its composition. The bioreactor is intended for use in the Laboratory of Experimental Biophotonics. This laboratory is equipped with coherence-controlled holographic microscope (CCHM), which uses quantitative phase imaging (QPI) method. Thus, the bioreactor is adapted to the current requirements of this laboratory and optical elements of the bioreactor meet the requirements of the QPI method. This text specifies the cultivation conditions of the living cells and summarizes, how the conditions could be controlled in the live cell microscopy. Next some commercially available culture devices are described and assessed, whether they are convenient for the~use in Laboratory of Experimental Biophotonics. The crucial part of the thesis is the~design, construction and testing of the new bioreactor.
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Vliv kultivačních podmínek na kryoprezervaci půdních řas a sinic
Lukešová, Alena
Fundamental principles of both conservative and vitrification cryopreservation methods are presented, and factors affecting cryopreservation of algae and cyanobacteria are summarized. These factors include factors connected with the cryopreservation process itself as well as factors connected with pre- and post-cryopreservation manipulation with samples. Attention is paid to cultivation conditions, mainly to the temperature in the context of application of cold acclimation before cryopreservation. Both literature data and our experience indicate that the response of soil algae and cyanobacteria to particular factors is species or strain specific and optimization of condition can enhance post-cryopreservation viability of cryopreservation recalcitrant strain.
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